Endocrine Abstracts

ObjectiveThe aim of this investigation is to study the redox status of osteoblasts in response to glucose and bacterial lipopolysaccharide (LPS) and their response to minocycline, in a simulated diabetogenic model, using the steroid marker 5 alpha- dihydrotestosterone (DHT)MethodMonolayer cultures of MG63 osteoblasts were established in Eagle’s MEM incubated for 24 h with radiolabelled testosterone, effe...

ObjectiveThis study investigates the oxidative effects of tumour necrosis factor alpha (TNF) and glucose (G) in a cell culture model of osteoblasts and the modifying role of minocycline (M), using a steroid marker of wound healing (5 alpha- dihydrotestosterone: DHT). This model simulates chronic inflammation and hyperglycaemia, relevant to chronic inflammatory periodontal disease in uncontrolled diabetics and prospects of adjunctive management, using min...

Aims This is an investigation of potential oxidative effects of AGE in response to the antioxidant glutathione in cultured human gingival fibroblasts, using radiolabelled steroids as substrates and their product 5 alpha dihydrotestosterone (DHT) as a marker of wound healing.Methods Confluent monolayer cultures of human gingival fibroblasts were incubated with 14C-Testosterone and 14C-4-androstenedione respect...

The anabolic role of androgens in growth and regeneration, has similar implications in periodontal tissues. Studies suggest that androgens and growth factors could affect activity of the enzyme alkaline phosphatase, being closely linked to anabolic effects on tissues matrices. Nicotine has detrimental effects on healing. One of the mechanisms involved is impairment of alkaline phosphatase activity. The aim of this investigation is to study the response of nicotine to platelet ...

Physiologically active androgens and growth factors contribute to healing responses by a variety of mechanisms, including stimulation of enzymes associated with matrix turnover. Some of these effects may be partially negated by nicotine. Both androgens and growth factors are able to compensate for oxidative effects induced by nicotine, being a significant deterrent to wound healing. The aim of this investigation is to establish the metabolic yields of androgen biomarkers in re...

Increased severity of periodontal disease has been reported in post-menopausal oestrogen deficient subjects, made worse by smoking. It was relevant to investigate this concept, using fibroblasts in culture and extrapolating the yields of androgen metabolites as an index of healing. The aim of this study is to establish the effects of nicotine (N), oestradiol (O) and the alkaline phosphatase inhibitor levamisole (L) on androgen metabolism in human gingival fibroblasts in this c...

Glucocorticoids modulate the effects of other hormones and also mediate cell function. Dexamethasone, in common with androgen metabolites, has been shown to increase alkaline phosphatase activity and bone specific marker proteins The aim of this investigation is to establish an androgen responsive element to dexamethasone as an index of regenerative potential in the periodontium, using the alkaline phosphatase inhibitor levamisole (L). 4 cell lines of oral periosteal fibroblas...

The aim of this investigation is to establish the effect of alkaline phosphatase on androgen metabolism in human gingival fibroblasts (obtained local Ethical Committee approval) and its possible mediation by the alkaline phosphatase inhibitor levamisole and nicotine, using radiolabelled testosterone as substrate. Monolayer cultures of fibroblasts were established in Eagle's MEM. Duplicate incubations were performed in multiwell plates with 14C-testosterone as substrate, in the...

Increased severity of periodontal disease has been reported in post-menopausal oestrogen deficient subjects, made worse by smoking. It was relevant to investigate this concept, using fibroblasts in culture and extrapolating the yields of androgen metabolites as an index of healing. The aim of this study is to establish the effects of nicotine (N), oestradiol (O) and the alkaline phosphatase inhibitor levamisole (L) on androgen metabolism in human gingival fibroblasts in this c...

The aim of this investigation is to study androgen metabolism in human gingival fibroblasts exposed to a hyperglycaemic environment and their response to insulin, the antagonist glucagon and nicotine; this may be extrapolated to their metabolic responses in vivo, in uncontrolled diabetes (obtained local Ethical Committee approval). Duplicate incubations of four cell lines of monolayer cultures of fibroblasts were performed in Eagle's MEM with radiolabelled testosterone as subs...